P53 is a tumor-suppressing gene expressed in many tissue types. It is involved in regulating cell replication, growth, and apoptosis. It can bind to the SV40T and MDM2 antigens, as well as the human papilloma virus’s E6 protein. It can sense damage to the DNA and may be able to help with repairs, as well. Mutations of the P53 antigen can be found in malignant tumors of the ovaries, bladder, breast, lung, colon and melanoma.
It is designed to be used for research and has a clone of SPM 514. The immunogen is the recombinant human protein of P53. It has no determined epitope and a molecular weight of 53kDa.
Applications
The P53 antibody is designed for Immunohistochemistry applications. To start preparing specimens, you should use paraffin-embedded or formalin-fixed tissues. Likewise, deparaffinized slides should be used and cleaned with xylene or an alternative.
You’ll find pre-diluted formats, as well as concentrated formats of this product. If you choose the concentrated version, you should dilute it using a ratio of 1:400, though this is an estimate. Your particular needs may change depending on the protocols and methods used.
To retrieve the antigen, you will need to boil a tissue section in a 10mM citrate buffer with a pH of 6.0 for at least 10 minutes. Likewise, it should be allowed to cool to room temperature for 20 minutes before incubating at room temperature for 30 minutes.
The positive control is the colon carcinoma, and cellular localization is in the nucleus.
Purchase Options
You will find a 7.0 ml pre-diluted formula of the P53 antibody. It is held in a BSA buffer with a pH of 7.6. You can also find three sizes of the concentrated formula, including 0.1 ml, 1.0 ml, and 0.5 ml.
The P53 antibody can help you research various antigens. Visit Spring Bioscience to learn more.
